Responsable: Sebastián Chávez de Diego
Tipo de Proyecto/Ayuda: Plan Nacional del 2010
Referencia: BFU2010-21975-C03-03
Fecha de Inicio: 01-01-2011
Fecha de Finalización: 31-07-2014

Empresa/Organismo financiador/es:

• Ministerio de Ciencia e Innovación

Equipo:

• Investigadores:
  • María Dolores de Miguel Jiménez
  • María de la Cruz Muñoz Centeno
• Personal Investigador en Formación:
  • Lidia Delgado Ramos (alta: 01/09/2011)
• Becarios:
  • Gonzalo Millán Zambrano (alta: 01/09/2011)

Contratados:

• Investigadores:
  • María Dolores de Miguel Jiménez
  • Xenia Peñate Salas

Resumen del proyecto:

It is currently clear that control of gene expression in eukaryotes is a multilayer phenomenon that affects all individual steps in transcription: from initiation to mRNA stability. The complexity of phenomenon needs new approaches, including genomic ones, to be deciphered. This can only be performed in depth using a model organism such the yeast as S. cerevisiae.

We plan to implement and to improve genomic and genetic techniques in yeast to study transcription in vivo in order to clarify the functional networks controlling genes and genome-wide transcription.

This coordinated project continues the collaboration initiated seven years ago by three research groups with complementary approaches and similar interest in gene transcription. The general objectives of this proposal are:

• To define the mechanisms that regulate gene expression during transcription elongation, in particular the RNA pol II arrest in ribosomal protein and mitochondria-related genes.
• To extend the study to human cells in culture.
• To study of the interplay between transcription rate and mRNA stability and the regulatory pathways controlling it.
• Functional definition of specific RNA Polymerase II domains and characterisation of their interactions with transcriptional modulators
• To determine global and particular effects of the physiological conditions on transcription rates.
• The analysis of the connection between some known elements of the transcriptional machinery (Rpb5) and other new elements (Rep1, Bud27).
• To determine the transcriptional effects of the free histones level.