Responsable: José María Romero Rodríguez
Tipo de Proyecto/Ayuda: Plan Nacional del 2005
Referencia: BIO2005-04916
Fecha de Inicio: 31-12-2005
Fecha de Finalización: 31-12-2008

Empresa/Organismo financiador/es:

• Ministerio de Educación y Ciencia

Equipo:

• Contratados:
  • Teresa Ruiz Pérez

Contratados:

• Investigadores:
  • Tania García Becerra
  • Alicia Orea García
  • Tiziana Ventriglia
• Técnicos/Personal Administrativo:
  • Raquel González Palma
  • Francisco Javier Guerle González

Resumen del proyecto:

Starch is the main form of carbon reserve in higher plants. The interest of this reserve polymer is due to a high production, its wide distribution, and for being basic for nutrition and for industry. ADP-Glucose Pyrophosphorylase (ADPGPPase) catalyzes the rate limiting and regulated step in starch synthesis. We will proceed to determine the contribution to starch síntesis of the 6 ADPGPPase isoforms present in Arabidopsis thaliana by characterizing T-DNA mutants. The elements and factors that mediate the transcriptional sugar regulation of ApL3 and ApL4 genes will be studied by different approaches. On the other hand, the role of genes involved in floral induction on starch and sucrose synthesis will be analyzed at level of ADPGPPase and Sucrose Phosphate Synthase (SPS) by studying the role of the diferent isoforms of either enzyme present in Arabidopsis.  Finally, we will characterize and select wheat transgenic lines generated in our lab. We have already introduced constructs to increase the expression of ADPGPPase and thus starch content. The transgenic wheat lines will be analyzed for increased ADPGPPase activity and starch levels.